Journal: The Journal of Neuroscience

Article Title: Octopamine Modulates the Axons of Modulatory Projection Neurons

doi: 10.1523/JNEUROSCI.2078-04.2004

Figure Lengend Snippet: Synaptic vesicle staining surrounds ion axon collaterals at the level of the stn-son junction. Axons were filled with neurobiotin, which was then visualized by streptavidin coupled to Cy5 (B, C, green), and synaptic vesicle-containing structures were revealed by anti-synaptotagmin staining visualized with anti-rabbit IgG coupled to AlexaFluor 488 (B, C, red). A, Schematic of the preparation showing the origin and the trajectory of the neurobiotin-filled axons. The black and white boxes represent the location of the pictures presented in B and C. B, Low magnification of the neurobiotin staining (left panel, green) and the synaptotagmin staining (middle panel, red) at the level of the stn-son junction. C, High-magnification pictures of the region surrounded in B. Note that the synaptotagmin staining is specifically located around the fine axon processes and not around the main axon branches.

Article Snippet: Preparations were then washed four to five times in PTA and incubated overnight in 1:400 anti-rabbit IgG coupled to AlexaFluor 488 (Molecular Probes, Eugene, OR) and 1:400 streptavidin coupled to Cy5 (Jackson ImmunoResearch, West Grove, PA).

Techniques: Staining